NotchBio lab platform overview

NotchBio is a guided bulk RNA-seq workspace. You bring raw FASTQ files from your computer, a public archive, Illumina BaseSpace, or Box; organize them into projects and experimental groups; then launch a reproducible analysis run.

The end-to-end path

1. Sign in and review your account. Set a password if you use a social sign-in, and confirm your profile details.
2. Acquire FASTQ data. Upload files directly or import from NCBI/ENA/DDBJ archives, Illumina BaseSpace, or Box.
3. Create a project. Select the organism once, then add datasets and collaborators.
4. Create experimental groups. Put biological replicates into groups such as Control and Treatment.
5. Configure and launch a run. Choose the baseline, cleanup profile, quantification method, and optional pathway analyses.
6. Review Results. Use the overview, gene expression, sample quality, and pathway views to understand what changed.

Core terms

Term	Meaning
Dataset	A set of imported or uploaded sequencing files stored in your account.
Sample	One biological or technical sample, usually represented by one single-end file or an R1/R2 pair.
Project	The workspace that contains samples, groups, collaborators, and runs.
Group	A biological condition used to define comparisons.
Run	One immutable analysis configuration and its processing outputs.
Result	A plot, table, or report produced by a completed run.

Choose your next task

• Manage your account
• Import public archive data
• Create a project
• Prepare samples and groups
• Configure a run
• Understand results