Quality control

Quality control is the first processing stage. NotchBio checks the raw reads before cleanup so you can distinguish a sequencing issue from a preprocessing choice.

Key metrics

• Total reads and read length
• Per-base quality and Q30 rate
• GC-content distribution
• Adapter and overrepresented-sequence content

Review metrics per sample and compare them with group labels. No single threshold proves that a sample is usable; investigate outliers alongside the preprocessing impact shown later in Results.

If quality needs attention

Choose a cleanup profile in the run wizard and inspect how many reads survive. If the issue is severe or sample-specific, pause before interpreting differential expression and consult the support team.